Part:BBa_K1356002:Experience

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Applications of BBa_K1356002

Dispersin B has been repeatedly characterized against pathogenic bacterial biofilms found in the medical field. However, given the unique nature of each biofilm it was necessary to characterize its efficacy against biofilms found in activated sludge processors. Dispersin B was tested alongside BSA protein and a glycerol control in a qualitative test to determine inhibition of biofilm attachment to micro centrifuge tubes. 15 tubes were given a 50ul sample of biofilm taken from the Silver Creek Wastewater Reclamation facility near Park City Utah. We added 12.5ul of Dispersin B protein suspended in glycerol solution to 5 of the tubes, BSA protein suspended in glycerol solution as a protein control to another 5, and a blank sample of glycerol control to the final 5 at the same time. These mixtures were allowed to set for 6 days to allow to biofilm to attach. At the end of the period each tube was inverted and given two firm taps. In comparison with the controls, the Dispersin B mixture was seen qualitatively to inhibit attachment of the biofilm. In the DispersinB samples, the biofilm dislodged was broken up into far smaller fragments, while the biofilm in the control groups consistently exhibited greater attachment to itself and the tubes.

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